Prevalence of Carbapenem-resistant bugs in rectal swabs from patients admitted in Medical ICU

Dr Anuj ParkashWritten by | MICROBIOLOGY, PATHOLOGY

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With more people being exposed to antibiotics, intestinal flora faces constant pressure of antibiotic selection, which has resulted in the emergence of multidrug resistant strains. This surveillance study was undertaken to investigate the carriage of carbapenem-resistant Enterobacteriaceae (CRE) in the gastrointestinal tract among patients admitted in Medical Intensive Care Unit (MICU) in a cancer hospital in Delhi.

The 178 rectal swab samples were collected from patients and 31 from the staff of MICU. Twenty-nine (13.8 per cent) isolates demonstrated carbapenemase activity among 209 screened isolates. Out of the 29, thirteen patients were carriers and none of the clinical samples showed any growth and no correlation was observed between the rectal carriage and clinical samples in 04 patients. Twelve patients showed similarity i.e. rectal swabs and clinical samples showed similar carbapenemase activity.

These 29 samples were further evaluated by Real time PCR for Klebsiella pneumoniae carbapenemase (KPC) and New Delhi metallo-β-lactamase (NDM) genes, as Real time PCR has been considered valuable tool in proving the endogenous spread.

Majority of the strains expressed NDM genes and KPC was not expressed at all.  Out of 12 paired samples, nine samples showed similarity i.e. NDM gene expression was same proving endogenous route of spread.

Conclusion- Screening for carriage of CRE in stools of patients undergoing elective or emergency gastrointestinal surgical procedures can be done. This guides clinicians about the gut colonization of multidrug-resistant Enterobacteriaceae, as these groups of patients, are at risk of possible endogenous infection. Hospitals should consider enhanced control measures such as contact precautions, isolation or cohorts, and dedicated nursing for patients who are CRE-positive, i.e. patients confirmed with digestive tract carriage of CRE or confirmed infection with CRE.

Disclaimer: The picture used in this blog is for education purpose only with no commercial usage. 

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Last modified: 06/09/2017

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